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We hypothesized that the muscle protein anabolic resistance to amino acids occurs in older adults and that RET could overcome such anabolic resistance by enhancing mTORC1 signaling and MPSin skeletal muscle. Methods Subjects Twenty-one resistance-trained men (age = 24.9 ± 0.8years; height = 1.78 m; body mass = 68.6 ± 0.7 kg) were recruited from the community and volunteered to participate. All subjects were experienced competitive powerlifters that trained at a minimum of 3RM. Exclusion criteria for the current study included use of nutritional supplements, use of medications known to adversely affect training, and a history of a musculoskeletal disorder. Exclusion criteria for previous studies include the presence of any medical condition/condition that limits the physical performance of the subject which could hinder the ability to adequately perform a resistance exercise program, such as diabetes and/or cardiac disease. Subjects were randomly assigned by a non-blinded, computer-generated randomization sequence to either the resistance program training group or the matched control group by means of a computerized randomization scheme. Subjects who were not previously trained with a resistance exercise program were selected for this study based on the following: (1) the fact that an older, lean man is commonly seen in an environment where muscle protein synthesis is enhanced after resistance training; (2) the fact that the majority of older people are currently training in a resistance training program of one or two sessions/week with the goal of reducing body fat or muscle mass; and (3) the fact that an older, lean man is frequently seen performing a variety of weight bearing activities such as jumping, jumping jacks, and crunches in an environment in which muscle protein breakdown is minimized. Study design This 12-week resistance training program consisted of three 3-week, double-elimination, cross-over training blocks and a 3-week rest time between blocks. At the end of the study, all subjects performed a 12-week post-training strength test at one of 3 different intensities (95% of max). Subjects then trained a total of 4 cycles/week for the duration of the study. Body composition and strength measurements during the training program were collected by an experienced professional laboratory technician, in compliance with good laboratory practices and according to previously published guidelines8 and the standards established by the National Strength and Conditioning Association (NSCA). All muscle biopsies were collected prior to any physical resistance exercise testing and were subsequently subjected to immunohistochemical staining to detect MHC class IV bands. Muscle protein mass was measured before and after the 4-week resistance training program Related Article: